Importantly, treatment with mesenchymal stem cells mscs suppressed autoimmunity and restored salivary gland secretory function in both mouse models and ss patients. Background mesenchymal stem cells mscs have the potential to replace infarct scar, but the longterm effects are unknown. Allogeneic mesenchymal stem cell transplantation in seven. However, most current research on stem cell treatment for coronary heart disease has been restricted to the repair of necrotic myocardium. However, to date there are few data about allogeneic msc transplantation msct for patients with inflammatory bowel disease ibd. Mesenchymal stem cells mscs, due to their immunosuppressive. Mscs were given to three patients for graft failure. Transplantation of mesenchymal stem cells to enhance. Thus, in allogeneic stem cell transplantation, mscs may be used for hematopoiesis enhancement, as gvhd prophylaxis, and for the treatment of severe acute. Aug 22, 2002 allogeneic mesenchymal stem cell infusion for treatment of metachromatic leukodystrophy mld and hurler syndrome mpsih skip to main content thank you for visiting. Spleens were harvested 24 or 48 hr after injection and expression of early activation markers was analysed.
We studied one hypothetical mechanism, neoangiogenesis, using allogeneic mesenchymal stem cell transplantation in an ischaemic swine model. Tissuespecific engraftment after in utero transplantation. Umbilical cord derived mesenchymal stem cells are immunosuppressive. Improving human kidney function in renovascular disease. We postulate that mesenchymal stem cells have a potent immunosuppressive effect in vivo. When a transplant is successful, the donor stem cells can replace stem cells in the bone marrow. Allogeneic mesenchymal stem cell infusion for treatment of metachromatic leukodystrophy mld and hurler syndrome mpsih skip to. One of the benefits of allogeneic stem cell transplantation is that after the donated cells engraft in the patient, they create a new immune system. Allogeneic bone marrow mesenchymal stem cells transplantation for stabilizing and repairing of atherosclerotic ruptured plaque author links open overlay panel shunmiao fang a dayong du b yuntian li b xingli ge a pengtao qin a qinghua zhang b yang liu b. Autologous and allogeneic mesenchymal stem cells in organ tr. Here we reveal, for the first time, that bone marrow mesenchymal stem cells in sslike nodltj mice and human patients were defective in immunoregulatory functions. Additionally, mscs are also used as immunosuppressants in allogeneic organ transplantation.
Mesenchymal stem cells mscs differentiate into various mesenchymal lineages, including bone and cartilage. Immunobiology of human mesenchymal stem cells and future use. Intravenous allogeneic mesenchymal stem cells for nonischemic. Given the difficulty in treating ssc, we conducted this study to investigate the effect of combined plasmapheresis pe and allogeneic mesenchymal stem cells transplantation msct therapy on ssc. The immunomodulatory properties of mesenchymal stem cells mscs make them a promising alternative treatment. Research open access safety and tracking of intrathecal allogeneic mesenchymal stem cell transplantation in healthy and diseased horses danielle jaqueta barberini1, monica aleman2, fabio aristizabal3, mathieu spriet3, kaitlin c. Context mesenchymal stem cells mscs are under evaluation as a therapy for is. Sustained benefit from combined plasmapheresis and allogeneic. However, new studies also suggest that efficacy may be curtailed by sequestration in the lungs and early elimination. Outcome was evaluated by changes in the sle disease activity index sledai, serological features antinuclear antibodies and antidoublestranded dna. Allogeneic bone marrowderived mesenchymal stem cell therapy.
Outcome was evaluated by changes in the sle disease activity index sledai, serological features antinuclear antibodies and antidoublestranded dna antidsdna. Mesenchymal stem cells for the treatment of refractory pure red cell aplasia after major aboincompatible hematopoietic stem cell transplantation. We refer to two articles by duijvestein et al and ciccocioppo et al 1 2 in which the authors suggest that administration of autologous bone marrowderived mesenchymal stem cells mscs is safe and feasible in the treatment of refractory crohns disease cd. Request pdf mesenchymal stem cells in allogeneic transplantation graft failure and graftvshost disease gvhd remain significant obstacles to successful outcome in patients undergoing. Mesenchymal stromal cells in renal transplantation. Allogeneic mesenchymal stem cells stimulate cartilage. The efficacy of allogeneic hematopoietic stem cell transplantation for congenital skeletal and storage disorders is limited, and therefore we focused on mscs for. The capacity to suppress the regular processes of allogeneic rejection, have allowed the use of tissue mismatched cells as therapeutic approaches in regenerative medicine and as agents of immune deviation. Allogeneic mesenchymal stem cell transplantation in severe. Safety concerns regarding autologous andor allogeneic mscs that have recently been investigated include transient proinflammatory effects, influences on opportunistic infections and cancers. Rationale and design of a randomized controlled trial of allogeneic mesenchymal stem cells in patients with nonischemic cardiomyopathy. Here we analyzed the role of allogeneic msct to induce renal remission in patients with active and refractory lupus nephritis ln. Transplantation of ace2 mesenchymal stem cells improves. The umbilical cordderived mesenchymal stem cells ucmscs were cultured and expanded using the ucsci technology developed by the stem cell institute, vnuhcm university of science, ho chi minh city, viet nam.
The unique immunomodulatory properties of multipotent mesenchymal stromal cells mscs make them a promising candidate for cell therapy in organ transplantation. Mesenchymal stem cells mscs, also known as multipotent mesenchymal stromal cells are a nonhematopoietic adult stem cell population that are present in various tissues such as bone marrow, adipose tissue, synovial membrane, and others. Allogeneic mesenchymal stem cell transplantation in postinfarcted. Jul 19, 2017 systemic sclerosis ssc is an autoimmune disease involving the skin and several internal organs. This dose escalation study confirmed no significant cellular toxicity, but it showed improvement in renal oxygenation and glomerular filtration rate. Wang d1, zhang h, liang j, li x, feng x, wang h, hua b, liu b, lu l, gilkeson gs, silver rm, chen w, shi s, sun l. These cells have a high safety profile for clinical use, are amenable to allogeneic use since tissue. Allogeneic mesenchymal stem cell transplantation in severe and. Phase iii study of safety and preliminary efficacy of. Ex vivo expanded allogeneic mesenchymal stem cells with bone. Safety and tolerability study of allogeneic mesenchymal. Moreover, their immunosuppressive properties and low immunogenicity contribute to a reduced or weakened immune response elicited by the implantation of allogeneic mscs compared with other cell types.
Mesenchymal stem cells enhance nerve regeneration in a rat. Methods entry criteria included ischemic stroke 6 months prior and substantial impairment national institutes of health stroke scale score. Treatment of knee osteoarthritis with allogeneic bone. Allogeneic stem cell transplantation leukemia and lymphoma. A longterm followup study of allogeneic mesenchymal stem. Methods this is an openlabel and singlecenter clinical trial conducted from 2007 to. Allogeneic mesenchymal stem cells transplantation in.
Transplantation of ace2 mesenchymal stem cells improves the outcome of patients with covid19 pneumonia article pdf available in aging and disease 112. Mesenchymal stem cells are characterized morphologically by a small cell body with a few cell processes that are long and thin. In recent years, the repair of damaged myocardium by autologous transplantation using bone marrow stem cells has become a hot topic in cardiovascular research. A dose escalation study of adiposederived human mesenchymal stem cell msc therapy was studied in 21 subjects. Bone marrow mononuclear cells were separated by ficoll tianjin haoyang biotech, inc. Intravenous transplantation of allogeneic bone marrow. Allogeneic mesenchymal stem stromal cells mscs have been widely studied as an alternative cell source for regenerative medicine. We studied short and longterm effects of msc transplantation on left ventricular lv function in a rat myocardial infarction model. Mscs can be detected in recipients at extended time points, suggesting a lack of immune recognition and clearance in vivo 1. Allogeneic mesenchymal stem cell transplantation in postinfarcted rat myocardium. The involvement of mesenchymal stromal cells mscs in both the innate and adaptive immune systems is multiple and complex. Ten patients were enrolled in this trial of bmmsct. Various cell types are candidates for cell therapy in ischemic stroke from embryonic stem cells to neuronal cell lines, and clinical trials of neuronal differentiated tumor cell lines, neural progenitor cells from primordial porcine striatum, and autologous bone marrow.
Nov 20, 2015 allogeneic bone marrowderived mesenchymal stem cells mscs will be delivered intravenously at one of four doses. The challenges and promises of allogeneic mesenchymal stem cells. The authors evaluated the effectiveness of allogeneic adipose tissuederived mesenchymal stem cell sheets in a rat model of stroke. Most therapies available for this disease are symptomatic.
Feb, 2020 isolation of umbilical cordderived mesenchymal stem cells. This study aimed to determine the safety and efficacy of allogeneic mesenchymal stem cells transplantation msct in refractory rheumatoid arthritis ra. The role of mesenchymal stromal cells in allogeneic. Allogeneic means the cells come from another person a donor. A reduction in inflammatory markers including tumor necrosis factor.
Current treatments vary in their efficacy and safety. The objective of this study was to evaluate the safety and efficacy of allogeneic bone marrow mesenchymal stromal stem cell transplantation bmmsct for patients with ursodeoxycholic acid udcaresistant primary biliary cirrhosis pbc. Allogeneic mesenchymal stem cells mscs do not induce markers of t cell activation. Allogeneic mesenchymal stem cell transplantation for lupus. Isolation of umbilical cordderived mesenchymal stem cells. Stem cell transplantation and mesenchymal cells to treat. Immunogenicity of allogeneic mesenchymal stem cells schu. Medicinal signaling cells mscs previously known as mesenchymal stem cells are multipotent stromal cells that can differentiate into a variety of cell types, including osteoblasts bone cells, chondrocytes cartilage cells, myocytes muscle cells and adipocytes fat cells. The underlying hypothesis is that the ssc condition is caused by an abnormal immune homeostasis that can be restored by msct.
Recent studies have demonstrated that allogeneic bone marrow transplantation bmt ameliorates the skin blistering phenotype of recessive dystrophic epidermolysis bullosa rdeb patients, who are severely compromised by dysfunction of type vii collagen col7 securing the epidermal structure to the underlying dermis of the skin. Four patients with persistently active ra underwent msct. Objective to determine the safety and efficacy of allogeneic mesenchymal stem cell transplantation msct in refractory systemic lupus erythematosus sle. Allogeneic adiposederived mesenchymal stem cell therapy in. Objective to assess the safety and clinical efficacy of allogeneic mesenchymal stem cell transplantation msct in a smallscale pilot study with 10 patients with drugresistant polymyositis pm or dermatomyositis dm. This method involves the use of cell sheet layers and allows the transplantation of a large number of cells, locally and noninvasively. Allogeneic mesenchymal stem cell infusion for treatment of.
Understanding the immunological mechanisms of mesenchymal. Pdf transplantation of ace2 mesenchymal stem cells. Allogeneic mesenchymal stem cells do not elicit a proliferative response. Mesenchymal stem stromal cells induce the generation of novel il10dependent regulatory dendritic cells by socs3 activation. Shen j, tsai yt, dimarco nm, long ma, sun x, tang l. We transplanted mesenchymal stem cells in a patient with refractory progressive ms, and the disease course was stabilized after the transplantation. Seven patients underwent treatment with mesenchymal stem cells mscs, together with allogeneic hematopoietic stem cell transplantation hsct. Allogeneic mesenchymal stem cells were injected in the periinfarct area 1. However, some risks in stem cell transplantation still serve as high. Allogeneic umbilical cordderived mesenchymal stem cell transplantation for treating chronic obstructive pulmonary disease. Allogeneic mesenchymal stem cells transplantation for.
The field has evolved substantially over the past decade, with advances in patient and donor selection, stem cell sources, supportive care, prevention of complications and reducedtoxicity preparative regimens. May 31, 2007 seven patients underwent treatment with mesenchymal stem cells mscs, together with allogeneic hematopoietic stem cell transplantation hsct. Allogeneic adiposederived mesenchymal stem cell sheet that. Allogenic mesenchymal stem cells transplantation in. However, the initial choice of autologous over allogeneic hsct was mainly based on the lower. The efficacy of mesenchymal stem cells in bronchiolitis.
Intracardiac allogeneic mesenchymal stem cell transplantation. Mscs are cells in the body that can grow into different types of cells and respond to various environmental situations. Backgroundpurpose allogeneic mesenchymal stem cell transplantation msct has been shown to be clinically efficacious in the treatment of various autoimmune diseases. Finally, current approaches to allogeneic msc application are. A phase 2 study of allogeneic mesenchymal stromal cells for luminal. Immunogenicity of allogeneic mesenchymal stem cells transplanted. Mesenchymal stem cells mscs are multipotential nonhematopoietic progenitors and are capable of differentiating into several tissues of mesenchymal origin. The donated stem cells can come from either a related or an unrelated donor. The authors used a scaffoldfree cell sheet as a translational intervention.
Furthermore, mesenchymal stem cells mscs from bone marrow cells are thought to be immuneprivileged and have been successfully transplanted into hearts. This patient received two infusions of allogeneic msc, in order to improve a possible defective bm stroma, to stimulate. To address allogeneic cell therapy limitations, this study developed a new allogeneic stem cell sheet using human umbilical cord mesenchymal stem cells hucmsc that. Pdf allogeneic mesenchymal stem cell transplantation in. Pdf allogeneic mesenchymal stem cell transplantation in severe. A major role of bone marrow mesenchymal stem and stromal cells is to support the function and survival of hscs in the bone marrow. Mesenchymal stem cells in allogeneic transplantation.
The challenges and promises of allogeneic mesenchymal stem. Adult mesenchymal stem cells possess a remarkably diverse array of immunosuppressive characteristics. Mesenchymal stem cells mscs have multiple mechanisms of action that make. Methods a singlearm trial involving 10 patients with dmpm who were either refractory to standard treatment, or had severe systemic involvement. Allogeneic mesenchymal stem cell transplantation in.
Transplantation before the age of 2 years before the onset of irreversible neurological decline, favorably alters the natural history of the disease. The current study examined safety and preliminary efficacy estimates of intravenous allogeneic mesenchymal stem cells in this population. The cell body contains a large, round nucleus with a prominent nucleolus, which is surrounded by finely dispersed chromatin particles, giving the nucleus a clear appearance. Gvhd in the setting of allogeneic hematopoietic stem cell transplantation in. Transplantation of ace2 mesenchymal stem cells improves the. Engraftment of allogeneic mesenchymal stem cells in the. Hematopoietic stem cell transplantation an overview. Pdf allogenic mesenchymal stem cells transplantation in. The aim of this study was to evaluate the therapeutic efficacy and safety of allogeneic canine adipose mscs cadmscs in dogs with. Methods we randomized 30 patients with chronic knee pain unresponsive to conservative treatments and showing radiological evidence of. The full text of this article is available in pdf format.
Safety of mesenchymal stem cell transplantation biomedical. Pdf mesenchymal stem cells mscs are multipotential. The aim of this study was to evaluate the therapeutic efficacy and safety of allogeneic canine adipose mscs cadmscs in. Aug 12, 2011 this study aimed to determine the safety and efficacy of allogeneic mesenchymal stem cells transplantation msct in refractory rheumatoid arthritis ra.
Hematopoietic stem cell transplant hsct is the treatment of choice for patients with severe forms of mps i. Sep 17, 2019 mesenchymal stem cells mscs have multiple mechanisms of action that make these highly promising geroscience therapeutic candidates. No significant renal toxicity from cell therapy was shown. Tissuespecific engraftment after in utero transplantation of allogeneic mesenchymal stem cells into sheep fetuses.
Request pdf allogeneic mesenchymal stem cells transplantation in treatment of multiple sclerosis multiple sclerosis ms is a chronic autoimmune disorder of the central nervous system. The kind of stem cells we are studying are called allogeneic human mesenchymal stem cells or mscs. Canine atopic dermatitis ad is a common skin disease with a 1015 per cent prevalence. Allogeneic hematopoietic cell transplantation hct is a potentially curative procedure for a variety of hematologic malignancies. We performed a randomized, active control trial to assess the feasibility and safety of treating osteoarthritis with allogeneic mscs, and we obtain information regarding the efficacy of this treatment. Allogeneic msc transplantation in refractory sle 2269 crest. This study will explore safety and efficacy of allogeneic mesenchymal stem cells transplantation msct to treat patients with diagnosis of systemic sclerosisssc who have been resistant to multiple standard treatments. Outcome was evaluated by changes in the sle disease activity index sledai, serological features antinuclear antibodies and anti. Allogeneic umbilical cordderived mesenchymal stem cell. Aug 23, 2019 mesenchymal stem cell msc transplantation therapy appears to be an ideal strategy for repairing structural defects and restoring the functions of diseased tissues and organs. Allogeneic adiposederived mesenchymal stem cell therapy. Allogeneic stem cell transplantation involves transferring the stem cells from a healthy person the donor to the patients body after highintensity chemotherapy or radiation. Allogeneic bone marrow mesenchymal stem cells transplantation.
Here, we report a longterm followup study of allogeneic bone marrow andor umbilical cord msc transplantation msct in severe and drugrefractory systemic lupus erythematosus sle patients. The challenges and promises of allogeneic mesenchymal stem cells for use as a cell based therapy jun zhang, xiaowen huang, haijun wang, xiaoyan liu, tao zhang, yunchuan wang, and dahai hu department of burns and cutaneous surgery, xijing hospital, fourth military medical university, xian, shaanxi 710032 peoples republic of china. Allogeneic mesenchymal stem cell transplantation for. Relative to baseline, autologous but not allogeneic msc therapy was as sociated with.
Intravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head zhanghua li 2 1, wen liao, xilong cui, qiang zhao 3, ming liu 1, youhao chen 1, tianshu liu, nongle liu 33, fang wang, yang yi 4, ningsheng shao 3 1. Safety and tracking of intrathecal allogeneic mesenchymal. The importance of hla assessment in offtheshelf allogeneic. Human mesenchymal stem cell sheets in xenofree media for. Jul 19, 2017 most therapies available for this disease are symptomatic. Mesenchymal stem cells mscs are nonhematopoietic, multipotent stromal cells that can differentiate into a variety of cell types and. Although pig allogeneic mesenchymal stem cells are not. It may also provide the only longterm cure of the patients disease. Evaluation of the clinical benefits of intralacrimal transplantation of allogeneic mesenchymal stem cells mscs in dogs with mildmoderate and severe kcs was done. Mesenchymal stem cells mscs are ideal for cellbased therapy in various inflammatory diseases because of their immunosuppressive and tissue repair properties.1143 59 1538 1602 1649 107 905 1652 1688 732 434 77 1574 75 1096 648 386 1652 918 196 1446 218 1483 1308 1035 111 1241 1023 231 1091 1442